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Product Product ISO/TS 20224-8:2022
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Description Description

This document specifies a real-time polymerase chain reaction (real-time PCR) method for the qualitative detection of turkey-specific DNA derived from food and feed. It requires the extraction of an adequate amount of PCR amplifiable DNA from the relevant matrix and can be applied to the detection of turkey material derived from domestic turkey (Meleagris gallopavo) and wild turkey (Meleagris ocellata).

The target sequence is a partial fragment of the Meleagris gallopavo chromosome Z DNA sequence (i.e. GenBank accession number NC_015041.2), which is present as a single copy per haploid genome. The provided PCR assay for this target has an absolute limit of detection of five copies per reaction, with ≥ 95 % confidence at this concentration (LOD95 %).

Standard Number Standard Number ISO/TS 20224-8:2022
Title Title ISO/TS 20224-8:2022 Molecular biomarker analysis — Detection of animal-derived materials in foodstuffs and feedstuffs by real-time PCR — Part 8: Turkey DNA detection method
Status Status Published
Publication Date Publication Date 05 Oct 2022
Cross References Cross References
Descriptors Descriptors
ICS ICS 67.050
Committee Committee ISO/TC 34/SC 16 Horizontal methods for molecular biomarker analysis
ISBN ISBN
Publisher Publisher PECB Store
Format Format PDF
Delivery Delivery NO
Pages Pages 14
File Size File Size KB